Super-resolution Microscopy

STochastic Optical Reconstruction Microscopy (STORM) reconstructs a super-resolution fluorescence image by combining precise localization information for individual fluorophores in complex fluorescent specimens. N-STORM takes advantage of Nikon's powerful Ti2-E inverted microscope and applies high-accuracy, multi-color localization and reconstruction in three dimensions (xyz) to enable super-resolution imaging at tenfold the resolution of conventional light microscopes (up to approximately 20 nm in xy).

N-SIM E is a streamlined, affordable super-resolution system that provides double the resolution of conventional light microscopes. Combining N-SIM E and a confocal microscope allows you the flexibility to select a location in the confocal image, and easily switch to view it in super-resolution, enabling the acquisition of more detail.

  

 

 

Manufacturer:

 https://www.microscope.healthcare.nikon.com/products/super-resolution-microscopes/

Brochure:

https://d33b8x22mym97j.cloudfront.net/phase4/literature/Brochures/2ce-scmh-6s.pdf?mtime=20190705101921 (N-STORM)

https://d33b8x22mym97j.cloudfront.net/phase4/literature/Brochures/2ce-scmh-6s.pdf?mtime=20190705101921 (N-SIM E)

 

Specifications:   

 

N-STORM

XY resolution

Approximately 20 nm

Z-axis resolution

Approximately 50 nm

Imaging method

2D-STORM (normal mode and continuous mode)

3D-STORM (normal mode and continuous mode)

3D-Stack function

Max. field of view

80 µm x 80 µm

Acquisition speed

Up to 500 Hz

Multi-color imaging

Up to 3 colors

Compatible laser

LU-NV series laser unit: 488 nm, 561 nm, 647 nm

Laser combination: 405 nm/445 nm/488 nm/561 nm/647 nm,

Compatible microscope

Motorized inverted microscope ECLIPSE Ti2-E

Perfect Focus System

Motorized XY stage with encoders

Piezo Z stage

Objective

CFI SR HP Plan Apochromat Lambda S 100XC Sil (NA1.35)

CFI SR HP Apochromat TIRF 100XC Oil (NA 1.49)

CFI SR HP Apochromat TIRF 100XAC Oil (NA 1.49)

CFI HP Plan Apochromat VC 100X Oil (NA 1.40)

Camera

ORCA-Flash 4.0 sCMOS camera (Hamamatsu Photonics K.K.)

iXON Ultra DU-897U EMCCD camera (Andor Technology Ltd.)

Software

NIS-Elements Ar

Operating conditions

20 ℃ to 25 ℃ ( ± 0.5 ℃)

 

 

 

N-SIM E

Lateral resolution (FWHM of beads in xy)

115 nm*1 in 3D-SIM mode

86 nm*2 in TIRF-SIM mode

Axial resolution (FWHM of beads in z)

269 nm*1 in 3D-SIM mode

Image acquisition time

Up to 1 sec/frame (3D-SIM)

Imaging mode

3D-SIM (Reconstruction method: slice, stack)

Multi-color imaging

Up to 3 colors

Compatible laser

LU-NV series laser unit

488 nm, 561 nm, 640 nm

Compatible microscope

Motorized inverted microscope ECLIPSE Ti2-E

Perfect Focus System

Motorized XY stage with encoders

Motorized barrier filter wheel

Piezo Z stage

Objective

 

CFI SR HP Apochromat TIRF 100XC Oil (NA 1.49)

CFI SR HP Apochromat TIRF 100XAC Oil (NA 1.49)

CFI SR Plan Apochromat IR 60XC WI (NA 1.27)

CFI SR Plan Apochromat IR 60XAC WI (NA 1.27)

CFI Plan Apochromat Lambda 60XC (NA 0.95)*2

CFI Plan Apochromat Lambda 40XC (NA 0.95)*2

Camera

ORCA-Flash 4.0 sCMOS camera

 (Hamamatsu Photonics K.K.)

Software

NIS-Elements Ar

Operating conditions

20 ℃ to 25 ℃ ( ± 0.5 ℃)

*1 These values are measured using 100 nm diameter beads excited by a 488 nm laser. Actual resolution is dependent on laser wavelength and optical configuration.

*2 Supports slice reconstruction 

 

Location: 112 room

Responsible: Wenjuan Liu & Qinglong Qiao 

Training required: Training given by Qinglong or Wenjuan.

Booking: Contact to Qinglong

Usages costs: 300 Yuan/h

 

 

 

(数据更新至2019年08月15日)

© 2019 Dalian Institute of Chemical Physics, CAS 

Molecular Probes & Fluorescence Imaging Group

All rights reserved.

Zhaochao Xu

Professer

457 Zhongshan Road, Dalian. 116023, P. R. China

Phone: + 86- 041184379648

Emai: zcxu@dicp.ac.cn