STochastic Optical Reconstruction Microscopy (STORM) reconstructs a super-resolution fluorescence image by combining precise localization information for individual fluorophores in complex fluorescent specimens. N-STORM takes advantage of Nikon's powerful Ti2-E inverted microscope and applies high-accuracy, multi-color localization and reconstruction in three dimensions (xyz) to enable super-resolution imaging at tenfold the resolution of conventional light microscopes (up to approximately 20 nm in xy).
N-SIM E is a streamlined, affordable super-resolution system that provides double the resolution of conventional light microscopes. Combining N-SIM E and a confocal microscope allows you the flexibility to select a location in the confocal image, and easily switch to view it in super-resolution, enabling the acquisition of more detail.
Manufacturer:
https://www.microscope.healthcare.nikon.com/products/super-resolution-microscopes/
Brochure:
Specifications:
N-STORM
XY resolution |
Approximately 20 nm |
Z-axis resolution |
Approximately 50 nm |
Imaging method |
2D-STORM (normal mode and continuous mode) 3D-STORM (normal mode and continuous mode) 3D-Stack function |
Max. field of view |
80 µm x 80 µm |
Acquisition speed |
Up to 500 Hz |
Multi-color imaging |
Up to 3 colors |
Compatible laser |
LU-NV series laser unit: 488 nm, 561 nm, 647 nm Laser combination: 405 nm/445 nm/488 nm/561 nm/647 nm, |
Compatible microscope |
Motorized inverted microscope ECLIPSE Ti2-E Perfect Focus System Motorized XY stage with encoders Piezo Z stage |
Objective |
CFI SR HP Plan Apochromat Lambda S 100XC Sil (NA1.35) CFI SR HP Apochromat TIRF 100XC Oil (NA 1.49) CFI SR HP Apochromat TIRF 100XAC Oil (NA 1.49) CFI HP Plan Apochromat VC 100X Oil (NA 1.40) |
Camera |
ORCA-Flash 4.0 sCMOS camera (Hamamatsu Photonics K.K.) iXON Ultra DU-897U EMCCD camera (Andor Technology Ltd.) |
Software |
NIS-Elements Ar |
Operating conditions |
20 ℃ to 25 ℃ ( ± 0.5 ℃) |
N-SIM E
Lateral resolution (FWHM of beads in xy) |
115 nm*1 in 3D-SIM mode 86 nm*2 in TIRF-SIM mode |
Axial resolution (FWHM of beads in z) |
269 nm*1 in 3D-SIM mode |
Image acquisition time |
Up to 1 sec/frame (3D-SIM) |
Imaging mode |
3D-SIM (Reconstruction method: slice, stack) |
Multi-color imaging |
Up to 3 colors |
Compatible laser |
LU-NV series laser unit 488 nm, 561 nm, 640 nm |
Compatible microscope |
Motorized inverted microscope ECLIPSE Ti2-E Perfect Focus System Motorized XY stage with encoders Motorized barrier filter wheel Piezo Z stage |
Objective |
CFI SR HP Apochromat TIRF 100XC Oil (NA 1.49) CFI SR HP Apochromat TIRF 100XAC Oil (NA 1.49) CFI SR Plan Apochromat IR 60XC WI (NA 1.27) CFI SR Plan Apochromat IR 60XAC WI (NA 1.27) CFI Plan Apochromat Lambda 60XC (NA 0.95)*2 CFI Plan Apochromat Lambda 40XC (NA 0.95)*2 |
Camera |
ORCA-Flash 4.0 sCMOS camera (Hamamatsu Photonics K.K.) |
Software |
NIS-Elements Ar |
Operating conditions |
20 ℃ to 25 ℃ ( ± 0.5 ℃) |
*1 These values are measured using 100 nm diameter beads excited by a 488 nm laser. Actual resolution is dependent on laser wavelength and optical configuration.
*2 Supports slice reconstruction
Location: 112 room
Responsible: Wenjuan Liu & Qinglong Qiao
Training required: Training given by Qinglong or Wenjuan.
Booking: Contact to Qinglong
Usages costs: 300 Yuan/h
© 2019 Dalian Institute of Chemical Physics, CAS
Molecular Probes & Fluorescence Imaging Group
All rights reserved. 辽ICP备05000861号
Professer
457 Zhongshan Road, Dalian. 116023, P. R. China
Phone: + 86- 041184379648
Emai: zcxu@dicp.ac.cn