Fluorescence Microscopy

Andor live cell confocal imaging platform integrated with CSU-W1 Spinning Disk Field Scanning Confocal System, iXon Ultra EMCCD and temperature control system, allow users flexible and powerful systems for a wide range of imaging applications. By using a disk containing microlens arrays in combination with the Nipkow disk, rotated to scan the entire field of view at high speeds, thus, making it possible to view confocal fluorescent images in real-time through the eyepiece of the CSU head. As compared to conventional single point scanning, multi beam scanning by the CSU requires a significantly low level of light intensity per unit area, which results in significantly reduced photo bleaching and phototoxicity in live cells. And then EMCCD camera has many technical advantages such as high sensitivity, high resolution, and high readout speed, which further enhances the imaging capabilities of Andorra Live Cell Confocal Imaging Platform.

Manufacturer:

CSU-W1 Confocal Scanner Unit

https://www.microscope.healthcare.nikon.com/products/confocal-microscopes/csu-series/csu-w1

iXon EMCCD Cameras

https://andor.oxinst.com/products/ixon-emccd-cameras

Specifications:

Confocal/wide field wavelength range	λex：400-800nm λem：420-850nm
Confocal speed	400fps
Laser wavelength	405 nm，488 nm，561 nm，640 nm
TIRF Imaging parameters	405 nm，488 nm，561 nm，640 nm；60×Objective lens
Wide field input power	2W maximum for combined wavelength
Camera	EMCCD electron multiplying charge coupled device
Confocal pinhole	25μ and 40μm
Active synchronization	Laser irradiation synchronized camera exposure to minimize phototoxicity and photobleaching
Output Power	500mW/1W
Horizontal resolution	Limited by diffraction within 19mm diagonal
Eyepiece magnification	10×
Objective magnification	10× 20× 40× 60×(TIRF) 100×
Size	31.2 × 30.7 × 19.3 (cm)

Location: 112 room

Responsible: Wei Zhou & Qinglong Qiao

Training required: Training given by Qinglong or Wei.

Booking: http://samp.cas.cn/admin.jsp (CAS)

Contact to Qinglong (Others)

Usages costs: 300 Yuan/h

The Olympus FV1000 MPE confocal laser scanning microscope offers conventional confocal laser scanning of samples on slide. Supports multicolor fluorescent studies for imaging of living, whole mount or thickly sliced specimens. Dynamic biological processes can be imaged hundreds of micrometers within living cells and tissues. Low magnification lens and long working distance stage allow imaging of large samples. Provides support for applications where phototoxicity/photobleaching is a concern such as time course studies of living cells and tissues.

This microscope equipped with electric stage, CO2 heating incubation system, 5 lasers, 4 detection channels and 1 transmitted light detection channel. Single photon confocal microscope system was built on an Olympus inverted microscope IX81. Two-photon confocal microscope system was built on the Olympus research-grade upright microscope BX61WI.

Manufacturer:

https://www.olympus-lifescience.com.cn/zh/support/obsolete/

Specifications:

Single photon laser unit	Laser: 405nm, 458nm, 488nm, 543nm, 635nm Motorized microscopes: Olympus IX81
Multi photon laser unit	Mai Tai (Spectra-Physics) 690nm-1040nm Motorized microscopes: Olympus BX61WI
Scanning method	Light deflection via 2 galvanometer scanning mirrors
Scanning speed	(pixel time): 2 μs — 5ms
Pixel size	64×64 — 2048×2048 pixels
High-speed scanning mode	16 frames/sec (256×256)
Eyepiece magnification	10×
Objective magnification	5× 10× 20× 40× 60× 100× (IX81) 5× 10× 20× 25× 40× (BX61WI)
Dimensions	Time, Z, (wavelength) (or any combination thereof)
Line scan	straight line (includes rotation), free line, point XY scan
Software	FV10-ASW
Required installation environment	Room temperature: 20°C — 25°C ± 1°C, humidity: 60% or less, dust level: Class 10000, requires continuous (24-hour) power supply
Power source environment	AC100-120/220-240V 60VA

Location: 112 room

Responsible: Ning Xu & Qinglong Qiao

Training required: Training given by Qinglong or Ning.

Booking: http://samp.cas.cn/admin.jsp (CAS)

Contact to Qinglong (Others)

Usages costs: 300 Yuan/h

Olympus IX73 inverted microscope is usually used for imaging living cells. It can perform fast fluorescence imaging, differential interference phase contrast imaging, phase contrast imaging and bright field imaging on cells according to different requirements of users. It has flexibly module configuration and high imaging resolution .

Manufacturer:

https://www.olympus-lifescience.com.cn/en/microscopes/inverted/ix73/

Maunal Book

https://www.manualslib.com/manual/662746/Olympus-Ix71.html?page=2#manual

Specifications:

Observation Method	Fluorescence (Ultraviolet/Blue/Green/Excitation), Differential Interference Contrast (DIC), Phase Contrast, Brightfield
Light source	Mercury spectrum lamp
Objective	4×, 10×, 20×, 40×
Optical system	UIS2

Location: 112 room

Responsible: Qinglong Qiao

Training required: Training given by Qinglong

Booking:Contact to Qinglong

Usages costs: 300 Yuan/h

（数据更新至2019年10月31日）