Fluorescence Microscopy

 

Andor live cell confocal imaging platform integrated with CSU-W1 Spinning Disk Field Scanning Confocal System, iXon Ultra EMCCD and temperature control system, allow users flexible and powerful systems for a wide range of imaging applications. By using a disk containing microlens arrays in combination with the Nipkow disk, rotated to scan the entire field of view at high speeds, thus, making it possible to view confocal fluorescent images in real-time through the eyepiece of the CSU head. As compared to conventional single point scanning, multi beam scanning by the CSU requires a significantly low level of light intensity per unit area, which results in significantly reduced photo bleaching and phototoxicity in live cells. And then EMCCD camera has many technical advantages such as high sensitivity, high resolution, and high readout speed, which further enhances the imaging capabilities of Andorra Live Cell Confocal Imaging Platform.

Manufacturer:

CSU-W1 Confocal Scanner Unit

https://www.microscope.healthcare.nikon.com/products/confocal-microscopes/csu-series/csu-w1

iXon EMCCD Cameras

https://andor.oxinst.com/products/ixon-emccd-cameras

  

Specifications:  

Confocal/wide field wavelength range

λex:400-800nm

λem:420-850nm

Confocal speed

400fps

Laser wavelength

405 nm,488 nm,561 nm,640 nm

TIRF Imaging parameters

405 nm,488 nm,561 nm,640 nm;60×Objective lens

Wide field input power

2W maximum for combined wavelength

Camera

EMCCD electron multiplying charge coupled device

Confocal pinhole

25μ and 40μm

Active synchronization

Laser irradiation synchronized camera exposure to minimize phototoxicity and photobleaching

Output Power

500mW/1W

Horizontal resolution

Limited by diffraction within 19mm diagonal

Eyepiece magnification

10×

Objective magnification

10× 20× 40× 60×(TIRF) 100×

Size

31.2 × 30.7 × 19.3 (cm)

 

Location: 112 room

Responsible: Wei Zhou & Qinglong Qiao

Training required: Training given by Qinglong or Wei.

Booking: http://samp.cas.cn/admin.jsp  (CAS)

                Contact to Qinglong (Others)

Usages costs: 300 Yuan/h

 

 

The Olympus FV1000 MPE confocal laser scanning microscope offers conventional confocal laser scanning of samples on slide. Supports multicolor fluorescent studies for imaging of living, whole mount or thickly sliced specimens. Dynamic biological processes can be imaged hundreds of micrometers within living cells and tissues. Low magnification lens and long working distance stage allow imaging of large samples. Provides support for applications where phototoxicity/photobleaching is a concern such as time course studies of living cells and tissues.

This microscope equipped with electric stage, CO2 heating incubation system, 5 lasers, 4 detection channels and 1 transmitted light detection channel. Single photon confocal microscope system was built on an Olympus inverted microscope IX81. Two-photon confocal microscope system was built on the Olympus research-grade upright microscope BX61WI.

 

 

Manufacturer:

https://www.olympus-lifescience.com.cn/zh/support/obsolete/

 

Specifications:

Single photon laser unit

Laser: 405nm, 458nm, 488nm, 543nm, 635nm

Motorized microscopes: Olympus IX81

Multi photon laser unit

Mai Tai (Spectra-Physics) 690nm-1040nm

Motorized microscopes: Olympus BX61WI

Scanning method

Light deflection via 2 galvanometer scanning mirrors

Scanning speed

(pixel time): 2 μs — 5ms

Pixel size

64×64 — 2048×2048 pixels

High-speed scanning mode

16 frames/sec (256×256)

Eyepiece magnification

10×

Objective magnification

5× 10× 20× 40× 60× 100× (IX81)

5× 10× 20× 25× 40× (BX61WI)

Dimensions

Time, Z, (wavelength) (or any combination thereof)

Line scan

straight line (includes rotation), free line, point XY scan

Software

FV10-ASW

Required installation environment

Room temperature: 20°C — 25°C ± 1°C, humidity: 60% or less, dust level: Class 10000, requires continuous (24-hour) power supply

Power source environment

AC100-120/220-240V 60VA

 

Location: 112 room

Responsible: Ning Xu & Qinglong Qiao

Training required: Training given by Qinglong or Ning.

Booking: http://samp.cas.cn/admin.jsp  (CAS)

                Contact to Qinglong (Others)

Usages costs: 300 Yuan/h

 

Olympus IX73 inverted microscope is usually used for imaging living cells. It can perform fast fluorescence imaging, differential interference phase contrast imaging, phase contrast imaging and bright field imaging on cells according to different requirements of users. It has flexibly module configuration and high imaging resolution .

 

 

 

 

Manufacturer:

https://www.olympus-lifescience.com.cn/en/microscopes/inverted/ix73/

Maunal Book

https://www.manualslib.com/manual/662746/Olympus-Ix71.html?page=2#manual

 

Specifications:

Observation Method

Fluorescence (Ultraviolet/Blue/Green/Excitation), Differential Interference Contrast (DIC), Phase Contrast, Brightfield

Light source

Mercury spectrum lamp

Objective

4×, 10×, 20×, 40×

Optical system

UIS2

Location: 112 room

Responsible:  Qinglong Qiao

Training required: Training given by Qinglong

Booking:Contact to Qinglong

Usages costs: 300 Yuan/h

 

 

 

 

(数据更新至2019年10月31日)

© 2019 Dalian Institute of Chemical Physics, CAS 

Molecular Probes & Fluorescence Imaging Group

All rights reserved.

Zhaochao Xu

Professer

457 Zhongshan Road, Dalian. 116023, P. R. China

Phone: + 86- 041184379648

Emai: zcxu@dicp.ac.cn