E2695 liquid chromatography host, including: dual pump head Quad gradient system, automatic injector, online plunger cleaning system, online degassing system; AllianceA series solvent bottle tray; 2998 diode array detector; Empower3 single system chromatography software; integrated hand screw connector; color spectrum column, etc. It is mainly used for rapid analysis of proteins, nucleic acids and small molecules in the fields of drug development and production, protein, food safety, environment, homeland security and petrochemical industry.
Manufacturer
https://www.waters.com/webassets/cms/library/docs/720000370zh.pdf
Manual Book
https://www.manualslib.com/products/Waters-Alliance-E2695-8983405.html
Specifications
Number of solvents: |
One to four |
Solvent conditioning |
Vacuum degas, two operating modes, four chambers, <500 µL internal volume per chamber |
Flow rate range |
0.01 to 10.000 mL/min (0.050 to 5.000 mL/min typical) in 0.001 mL/min increments |
Compressibility compensation |
Automatic and continuous |
Dwell volume (total system) |
≤1.145 mL |
Plunger seal wash |
Integral, active, programmable |
Gradient profiles |
11 gradient curves |
Dry prime/wet prime |
Automatic front panel control, System PREP function for automatic solvent(s) purge |
Flow ramping |
Time (0.01 to 30.00 min in 0.01 min increments) to reach maximum flow rate |
Maximum operating pressure |
5000 psi (345 bar [0.010 to 3.000 mL/min]) programmable upper and lower limits |
Composition range |
0.0% to 100.0%, in 0.1% increments |
Composition accuracy |
±0.5% absolute, independent of backpressure (proportioning valve pair test, [degassed methanol: methanol/propylparaben, 2.0 mL/min, 254 nm]) |
Composition precision |
≤0.15% RSD or ≤0.02 min SD, whichever is greater, based on retention time (60:40 degassed methanol/water dial-a-mix, 1.00 mL/min, six replicates, phenone mix, 254 nm) |
Flow precision |
≤0.075% RSD or ≤0.02 min SD, six replicates, based on retention time or volumetric measures (0.200 to 5.000 mL/min), isocratic premix |
Flow accuracy |
±1% or 10 µL/min, whichever is greater, 0.200 to 5.000 mL/min, (degassed methanol at 600 psi backpressure) |
Number of sample |
vials 120 vials, configured in five carousels of 24 vials each |
Number of sample injections |
1 to 99 injections per sample vial |
Sample delivery precision |
Typically <0.5% RSD, 5 to 80 µL (using standard 250 µL syringe), 60:40 degassed methanol/water dial-a-mix, 1 mL/min, six replicates, phenone mix, 254 nm);Typically <0.3% RSD, 5 to 60 µL (using 100 µL optional syringe), 70:30 degassed methanol/water dial-a-mix, 1 mL/min, six replicates, caffeine, 273 nm |
Injection accuracy |
±1 µL (±2%) (50 µL, N=6), sample: 100% degassed water, analytical solvent: 100% degassed methanol |
Standard sample vial |
2 mL |
Advanced operations |
Priority samples, auto additions, auto standards |
Injection volume range |
0.1 to 100.0 µL, standard; 0.1 to 2000.0 µL, with optional sample loop |
Injector linearity |
>0.999 coefficient of deviation (1.000 to 100.000 µL) |
Minimum sample required |
10 µL, using low volume inserts |
Sample temperature control |
4 to 40 °C, programmable in 1 °C increments, 60 minute time to temperature ±2 °C accuracy ±1 °C stability |
Column heater (optional) |
20 to 65 °C, in 1 °C increments (5 °C above ambient) |
Column heater/cooler (optional) |
Ambient minus 15 or 4 °C (whichever is greatest) up to 65 °C, in 1 °C increments |
Power requirements |
950 VA (maximum) |
Voltage range |
100 to 240 VAC |
Frequency |
50 to 60 Hz |
Operating temperature range |
4 to 40 °C |
Location: 107 room
Responsible: Wenchao Jiang
Training required: Training given by Wenchao.
Booking: Contact to Qinglong
Usages costs: 300 Yuan/h
The Prep 150 LC System, featuring ChromScope™ Software, is ideally suited for initial workflow criteria requiring rapid preparative separations of complex mixtures. Its versatility allows for manual or automated control to enable purification ranging from just a few samples to greater throughput. The Prep 150 LC System can easily scale up to Waters® AutoPurification™ LC System, offering full automation and higher levels of specificity and purity.
Manufacturer
https://www.waters.com/waters/zh_CN/Prep-150-LC-System/nav.htm?locale=zh_CN&cid=134727002
Manual Book
https://www.waters.com/webassets/cms/library/docs/720004637en.pdf
Specifications
2545 Quaternary System |
Rogrammable from 0 to 150 mL/min, in 0.01 increments |
2489 UV/Visible (UV/Vis) Detector |
|
Waters Fraction Collector III (WFC III) |
Cintillation vial (17 mm) or Scintillation vial (28 mm) |
Preparative column C18 |
C18 5 μm 10 ×150 mm; C18 5 μm 19 ×150 mm |
Location: 107 room
Responsible: Kai An
Training required: Training given by Kai.
Booking: Contact to Qinglong
Usages costs: 300 Yuan/h
© 2019 Dalian Institute of Chemical Physics, CAS
Molecular Probes & Fluorescence Imaging Group
All rights reserved. 辽ICP备05000861号
Professer
457 Zhongshan Road, Dalian. 116023, P. R. China
Phone: + 86- 041184379648
Emai: zcxu@dicp.ac.cn