FluoroMax-4 Compact Spectrofluorometer, the latest generation of the original, high performance tabletop fluorometer, which offers extended performance with detection of emission spectra out to 1700 nm and (TCSPC) Time Correlated Single Photon Counting lifetime measurements as short as 25ps. The FluoroMax series spectrofluorometers are ideal for measuring solid and liquid samples.
Manufacturer:
https://www.horiba.com/en_en/products/detail/action/show/Product/fluoromax-1576/
Manual Book：
Specifications:
Water Raman signal-to-noise ratio |
16000:1 (6000:1 FSD) |
Photon Counting for Ultimate Sensitivity |
Available |
Scanning speed |
up to 80 nm/second |
Software |
FluorEssence™ software for Windows® |
Record mode |
Method Files to recall complete experiment parameters |
accessories |
front measurement attachment microscopic fluorescence accessories polarization and anisotropic accessories optical fiber remote measurement accessories Time-correlated single-photon counting (TCSPC) accessory for lifetime determinations Phosphorimeter accessory for long-lived luminescence determinations |
Operating conditions |
20 ℃ to 25 ℃ ( ± 0.5 ℃) |
Location: 112 room
Responsible: Jin Li
Training required: Training given by Jin Li.
Booking: Contact to Jin Li
Usages costs: 300 Yuan/h
UV-visible spectrophotometer. The ultraviolet absorption spectrum of molecules is the absorption spectrum produced by the electron energy level transition after some groups in molecules absorb the ultraviolet radiation. It is a band spectrum that reflects information about some of the groups in the molecule. Qualitative analysis can be carried out by standard optical atlas in combination with other methods. According to Lambert-Beer law: A = εbc, (A is absorbance, ε is molar absorbance coefficient, b is liquid pool thickness, c is solution concentration), the solution can be quantitatively analyzed.
Manufacturer:
https://www.agilent.com/zh-cn/products/uv-vis-uv-vis-nir/uv-vis-uv-vis-nir-systems/cary-60-uv-vis
Manual Book:
https://www.agilent.com/cs/library/usermanuals/public/G6860-90001.pdf （English）
Specifications:
Monochromator |
high-performance Czerney-Turner monochromator |
Light source |
xenon pulse lamp, 3 billion times of flashing super long life |
Wavelength range |
190 ~ 1100nm |
Luminosity range |
±3.3Abs |
Photometric accuracy |
± 0.005a (with NIST930 filter at 1.0Abs) |
Baseline smoothness |
±0.001Abs |
Maximum scanning rate |
24,000nm /min |
Location: 107A
Responsible: Weiwei Liu
Training required: Training given by Weiwei.
Booking: Contact to Jin Li
Usages costs: 300 Yuan/h
Fluorescence spectrometer, also known as fluorescence spectrophotometer, is a qualitative and quantitative analysis of the instrument. Fluorescence emission/excitation spectra, phosphorescence spectra, chemical/bioluminescence, concentration determination, kinetics determination of measurable compounds.
Manufacturer:
Manual Book:
https://www.agilent.com/cs/library/usermanuals/public/1758.pdf
https://www.agilent.com/cs/library/usermanuals/public/1740.pdf
Specifications:
Light source |
long life flashing xenon lamp, flashing half peak width ≈2 microseconds, peak power equivalent to 75KW, life 20,000 hours |
Excitation/ emission monochromator |
Czemy-Tumer monochromator, F3.6 focal length 0.125m |
Filter |
(excitation filter: 250- 395nm; 335- 620 nm; 550- 1100 nm; 695- 1100 nm. Emission filter: 250- 395nm; 295- 1100 nm; 360- 1100 nm; 430- 1100 nm; 550- 1100 nm) |
Grating |
Excited state 30×35mm, 1200 line /mm, scintillation wavelength 370mm; Emission state 30×35mm, 1200 line /mm, scintillation wavelength is 440mm |
Detector |
high performance R928 photomultiplier detector, one for sample signal and one for reference signal. The voltage of photomultiplier tube can be divided into three levels according to the sample fluorescence intensity: low (400V, strong fluorescence), medium (600V, default value), high (800V, weak fluorescence), or manually input the self-selected value (400-1000v) |
Wavelength range |
excited state: 200-900nm, zero order optional; Emission state: 200-900nm zero level optional |
Wavelength accuracy |
±0.5nm (541.92nm) ±1.0nm (200-900nm) |
Detection limit |
< 1.0pm luciferin (standard pool); < 10pm fluorescent cable (40mul micropool); <25 pM QBS (standard pool) |
Band width |
excitation: 1.5, 2.5, 5,10,20 nm; Launch: 1.5, 2.5, 5,10,20 nm.Resolution: <1.5nm |
Scanning rate |
0.010-24000nm/min, increase by 0.15nm |
Average sampling time |
fluorescence 0.0125-999sec; Phosphorescent 1usec-10sec. Biological/chemiluminescence 40usec -10sec |
Repeat scan |
maximum repeat times 1000; Maximum cycle time 1000 minutes |
Location: 107A
Responsible: Weiwei Liu
Training required: Training given by Weiwei.
Booking: Contact to Jin Li
Usages costs: 300 Yuan/h
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Molecular Probes & Fluorescence Imaging Group
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Professer
457 Zhongshan Road, Dalian. 116023, P. R. China
Phone: + 86- 041184379648
Emai: zcxu@dicp.ac.cn